Changing the MySQL root password and changing some security options

So far I have been using MySQL with an empty root password for my local WP projects.

Will changing the MySQL root password from empty to something break parts of Devilbox?
Especially the selections made below at 'mysql_secure_installation' for 'Remove anonymous users?' and 'Disallow root login remotely?'.

With:

mysql_secure_installation
Change the root password? [Y/n] <-- y
New password: my-shiny-new-password-here
Re-enter new password: my-shiny-new-password-here
Remove anonymous users? [Y/n] <-- y
Disallow root login remotely? [Y/n] <-- y
Remove test database and access to it? [Y/n] <-- y
Reload privilege tables now? [Y/n] <-- y

or with :

mysqladmin -u root password my-shiny-new-password-here